Capsule staining: Principle, Reagents, Procedure and Result
Principle A capsule is non-ionic and very delicate structure that is secreted by the cell and that surrounds and adheres to the cell wall. It […]
Microbiology practical
Giemsa stain: Principle, procedure, result
Principle Giemsa stain is a Romanowsky stain that is widely used in parasitology to stain malaria and other blood parasites. In microbiology, the Giemsa technique […]
Kligler iron agar (KIA): composition, preparation, uses and interpretation of result
Kligler Iron Agar (KIA) Kligler iron agar (KIA) is a differential slope medium used to assist in the identification of salmonella, Shigella and other energetic […]
Glycerol saline transport medium: composition, preparation and uses
Glycerol saline transport medium is used to preserve Salmonella and Shigella species and Escherichia coli in facial specimens. It is not suitable for Vibrio cholera, Campylobacterspecies […]
Glucose broth, glucose phosphate peptone water and Glycerol broth
1. Glucose (dextrose) broth: Glucose broth is an enriched fluid medium used to culture a variety of nutritionally demanding microorganism. It is used in the […]
Dorset egg medium:composition, preparation and uses
Dorset egg medium Dorset egg medium is an enriched slope medium used to culture Corynebacterium diphtheriae to direct volutine granules. It is also used for […]
DNAse agar: composition, preparation and uses
DNAse agar is a plate biochemical medium used to identify Staphylococcus aureus from other species. It is most economically prepared from the dehydrated medium available […]
Deoxycholate citrate agar (DCA): composition, preparation and use
DCA Deoxycholate citrate agar (DCA) is a selective and differential medium used to isolate Salmonella and Shigella species. It can also be used to isolate […]
Cooked meat medium: composition, preparation and uses
Cooked meat medium Cooked meat medium is a nutritious fluid broth used in the enrichment, rapid growth and maintenance of micro organisms especially anaerobes. It […]
Christensen urea broth (maslen modified):composition, preparation and uses
Christensen modified urea broth Christensen modified urea broth is a biochemical fluid medium used to test microorganisms for urease production. It can be prepared from […]