Cetrimide agar: composition, preparation and uses
Centrimide agar Cetrimide agar is a selective plate medium used occasionally to isolate Pseudomonas species from a mixed bacterial flora. It inhibits the growth of […]
Microbiology practical
Cary-blair transport medium: composition, preparation and uses
Cary-blair transport medium Cary-blairsemisolid transport medium is used to preserve the viability of entericpathogens in faeal specimens Salmonella, Shigella, Vibrioand Yersinia enterocolittica survivewell in cary-blair […]
Butzler campylobacter medium: composition, preparation and uses
Butzler selective medium is used to isolate campylobacter jejuni and campylobacter coli from faeces. Butzlers Virion medium is recommended because it has been shown to […]
Types of blood agar: Crystal violet blood agar, Neomycin blood agar, Kanamycin blood agar and chocolate agar
1) Crystal violet blood agar: This is used as a selective medium for Streptococus pyogenes.At a concentration of 1 in 500000, crystal violet inhibits the […]
Blood agar: composition, preparation and uses
Blood agar: composition, preparation and uses Blood agar is used to grow a wide range of pathogenic organisms particularly those which do not grow in […]
Amies transport medium: composition, preparation and uses
Amies transport medium: composition, preparation and uses This is a transport medium used to preserve the viability of anaerobes such as Neisseria gonorrhea and other […]
Alkaline peptone water: composition, preparation and uses
Alkaline peptone water: composition, preparation and uses Alkaline peptone water is an enrichment medium used for Vibrio cholerae and other Vibrio The pH of the […]
Oxidation-Fermentation (OF) test: Objective, Principle, Procedure and Result
Objective to detect whether the organisms are oxidative or fermentative . Principle: The principle of this biochemical test is to detect the change in colour […]
TSI (Triple Sugar Iron) test: Objective, Principle, Procedure and Result
TSI (Triple Sugar Iron) test: Objective to check the ability of organism to ferment sugar (lactose, sucrose) and their ability to produce H2S gas. Principle […]
Nitrate reduction test: Objective, Principle, Procedure and Result
Nitrate reduction test: Objective to detect the ability of the organism to produce Nitrate reductase enzyme. to differentiate members of Enterobacteriaceae that produce enzyme nitrate […]