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Microbiology practical

Isolation of antibiotic producing microorganisms and Determination of antimicrobial spectrum of isolates

Isolation of antibiotic producing microorganisms and Determination of antimicrobial spectrum of isolates

February 7, 2024 Gaurab Karki 0

Isolation of antibiotic producing microorganisms and Determination of antimicrobial spectrum of isolates Principle: Soil is the prime storage of the microorganisms producing antibiotics which are […]

spore staining technique

Spore staining technique: principle, requirements and procedure

February 7, 2024 Gaurab Karki 0

Spore staining technique: principle, requirements and procedure Principle: Members of the anaerobic genera Clostridium and Desulfotomaculum and the aerobic genus Bacillus are examples of organisms […]

Cell culture contamination

Cell culture contamination

May 29, 2021 Gaurab Karki 0

Cell culture contamination: It is possible to describe a cell culture contaminant as any elements in the culture system that are unacceptable because of their […]

O-Nitrophenyl-β-D-Galactopyranoside (ONPG) test: Principle, Procedure and Results

O-Nitrophenyl-β-D-Galactopyranoside (ONPG) test: Principle, Procedure and Results

July 19, 2020 Gaurab Karki 0

Principle of ONPG test: Lactose is a disaccharide of glucose and galactose which is connected by a β-galactoside bond. Lactose fermenting bacteria consist of both […]

L-Pyrrolidonyl Arylamidase (PYR) test: Principle, Requirements, Procedure and Results

L-Pyrrolidonyl Arylamidase (PYR) test: Principle, Requirements, Procedure and Results

July 6, 2020 Gaurab Karki 0

Principle of PYR test: L-Pyrrolidonyl Arylamidase (PYR) test is one of the rapid tests employed for the identification of group a beta-hemolytic Streptococci and Enterococci […]

Decarboxylase test-Principle, Procedure and Results

July 6, 2020 Gaurab Karki 0

Principle of Decarboxylase test: Decarboxylases are a group of enzymes which acts by hydrolyzing an amino acid to form an amine. Decarboxylase test is mainly […]

Leucine Amino Peptidase (LAP) test: Principle, Procedure and Results interpretations

Leucine Amino Peptidase (LAP) test

July 6, 2020 Gaurab Karki 0

Principle: Leucine Amino Peptidase (LAP) test is one of the rapid tests which is employed for the detection of the enzyme leucine amino peptidase. The […]

Microdase (Modified Oxidase) test: Principle, Procedure and Results interpretation

Microdase (Modified Oxidase) test: Principle, Procedure and Results interpretation

July 6, 2020 Gaurab Karki 0

Principle: Microdase test is also termed as modified oxidase test. It is one of the rapid tests employed for the differentiation between Staphylococcus and Micrococcus […]

Novobiocin susceptibility test: Principle, Procedure and Results interpretations

July 6, 2020 Gaurab Karki 0

Principle: After E. coli, Staphylococcus saprophyticus is the second most causative organism for the Urinary tract infections (UTIs) in sexually active young women. Laboratory identification […]

Acetate utilization test: Principle, media composition, Procedure and Results

June 16, 2020 Gaurab Karki 0

Principle of Acetate utilization test: Acetate agar is employed to test an organism’s ability to utilize acetate. The medium consists of sodium acetate as the […]

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