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Protein and Amino acid tests

Pauly’s test: Objective, Principle, Reagents, Procedure and Result

June 29, 2018 Gaurab Karki 0

Pauly’s test: Objective, Principle, Reagents, Procedure and Result Objective: to detect aromatic amino acid  tyrosine or histidine Principle: Diazotized sulphanilic acid couples with amines, phenols […]

Adamkiewicz reaction (Hopkin’s-cole test): Objective, Principle, Reagents, Procedure and Result

June 29, 2018 Gaurab Karki 0

Adamkiewicz reaction (Hopkin’s-cole test): Objective, Principle, Reagents, Procedure and Result Objective: to detect amino acid tryptophan present in protein Principle: The indole group of tryptophan […]

Millon’s test: Objective, Principle, Reagents, Procedure and Result

April 25, 2018 Gaurab Karki 0

Millon’s test: Objective, Principle, Reagents, Procedure and Result Objective: to detect amino acid containing phenol group (hydroxyl group attached to benzene ring) ie. Tyrosine Principle of […]

Sakaguchi test: Objective, Principle, Reagents, Procedure and Result

April 25, 2018 Gaurab Karki 0

Sakaguchi test: Objective, Principle, Reagents, Procedure and Result Objective: to detect amino acid gauanidium group [R-NH-C= (NH2)2+-NH2]. i.e arginine Principle of Sakaguchi test: This test is specific […]

Lead sulfide test: Detection of amino acid containing sulfhydral group (-SH)

April 25, 2018 Gaurab Karki 0

Lead sulfide test: Detection of amino acid containing sulfhydral group (-SH) Objective: to detect amino acid containing sulfhydral group (-SH) ie cysteine Principle of lead […]

Qualitative Tests for amino acids and Proteins

April 25, 2018 Gaurab Karki 0

Qualitative Tests for amino acids and Proteins General test for amino acids and proteins: Following are the qualitative test to detect amino acids and protein […]

Xanthoproteic test: Objective, Principle, Reagents, Procedure and Result

April 23, 2018 Gaurab Karki 0

Xanthoproteic test: Objective, Principle, Reagents, Procedure and Result Objective: this test is used to differentiate aromatic amino acids which give positive result from other amino […]

Biuret test-Principle, Requirements, Reagents, Preparation, Procedure and Result

April 20, 2018 Gaurab Karki 0

Biuret test Principle of Biuret test: Biuret test is a general test for compounds having a peptide bond. Biuret is a compound formed by heating […]

Ninhydrin Test: Principle, Requirements, Procedure and Result

April 18, 2018 Gaurab Karki 0

Ninhydrin Test: Principle, Requirements, Procedure and Result Principle: This test is a general test and thus given by all amino acids. This test is due […]

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