Barfoed’s Test: Objective, Principle, Reagents, Procedure and Result Objective: to detect reducing sugar to distinguish monosaccharides from reducing disaccharides Principle of Barfoed’s test: Barfoed’s test […]
DNA extraction from Gram Positive bacteria (Staphylococcus aureus): materials required and protocol Materials required: Overnight culture of Staphylococcus aureus in LB TE buffer ( 10mM […]
DNA extraction from E. coli: Materials required: Overnight culture of coli in LB TE buffer ( 10mM tris.Cl, 1mM EDTA, pH 8.0) 10% SDS 1:1 […]
Molisch’s Test: Objectives, Principle, Reagents, Procedure and Result Objective: to identify carbohydrate from other biomolecules Principle of Molisch’s test: Molisch’s test is a general test […]
Fehling’s Test: Objective, Principle, Reagents, Procedure and Result Objective: to detect reducing sugar in a given solution Principle of Fehling’s test: Fehling’s test is one […]
Millon’s test: Objective, Principle, Reagents, Procedure and Result Objective: to detect amino acid containing phenol group (hydroxyl group attached to benzene ring) ie. Tyrosine Principle of […]
Sakaguchi test: Objective, Principle, Reagents, Procedure and Result Objective: to detect amino acid gauanidium group [R-NH-C= (NH2)2+-NH2]. i.e arginine Principle of Sakaguchi test: This test is specific […]
Lead sulfide test: Detection of amino acid containing sulfhydral group (-SH) Objective: to detect amino acid containing sulfhydral group (-SH) ie cysteine Principle of lead […]
Qualitative Tests for amino acids and Proteins General test for amino acids and proteins: Following are the qualitative test to detect amino acids and protein […]
Tests for specific carbohydrates: Seliwanoff’s test, Bial’s test and Iodine test 1. Seliwanoff’s test: Principle, reagents, procedure and result Principle of Seliwanoff’s test: Seliwanoff’s test […]
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