MRS Broth test: Principle, Composition, Procedure, and Results interpretation
Principle: MRS broth is a medium for the cultivation of lactobacilli and is specifically useful for a number of fastidious strains which is unable to […]
Biology Practical
Optochin susceptibility test: Principle, Procedure and Results interpretation
Principle of Optochin susceptibility test: Streptococcus pneumoniae is found generally on respiratory tract and has a hemolytic pattern identical to other alpha-hemolytic streptococci and lactobacilli. […]
4-Methylumbelliferyl-β-D-Glucuronide (MUG) Test
Principle of MUG test: About 97% strains of Escherichia coli and organisms as such Salmonella, Shigella, and Yersinia are capable of producing enzyme β-glucuronidase. MUG […]
Salmonella-Shigella Agar test: Principle, Composition, Procedure, Results interpretation
Principle: Salmonella-shigella (SS) agar is generally employed for the selective isolation and differentiation of Salmonella and Shigella in both clinical and nonclinical samples. However, SS […]
Satellitism test for identification of Haemophilus influenzae
Principle: Haemophilus spp. typically grows on chocolate agar as smooth, flat or convex buff, or slightly yellow colonies. Chocolate agar provides hemin (X factor) and […]
Phenylalanine deaminase test: Principle, Requirements, Procedure and Results interpretations
Principle: The phenylalanine deaminase (PDA) test is employed to differentiate among the urea- positive gram-negative bacilli on the basis of the ability of the microorganisms […]
Malonate test: Principle, Requirements, Procedure, and Result interpretations
Objective: To check whether the organism is capable of utilizing malonate as sole source of carbon and energy for its growth. To categorize organisms on […]
Dental caries susceptibility tests: Snyder test
Principle: Dental caries (tooth decay) is damage to enamel of tooth that develop into tiny holes or openings caused by bacteria. In course of the […]
Measurement of bacterial growth using UV spectrophotometer
Principle: The quantitative detection of the bacterial population is needed for various studies. There are basically two methods used for the counting of bacterial population […]
Enumeration of bacteria by plate count technique
Principle: There are various techniques/methodologies for the enumeration (counting) of bacteria in a given sample. A viable cell count permits to detect the number of […]