Beta Oxidation of fatty acid: steps and examples
Fatty acids Oxidation Oxidation of fatty acid (FA) releases large amount of energy. Oxidation of fatty acid occurs in mitochondria cell lacking mitochondria (e.g. RBC) […]
Methods of Cell disruption: Cell lysis methods
Cell disruption: The process of breaking apart of the cells in order to obtain the desired product which may be intracellular or periplasmic is called […]
Enzyme classification with examples
Classification of enzyme: on the basis of International Union of Biochemistry (IUB) system: Oxidoreductase Transferase Hydrolase Lyase Isomerase Ligase 1. Oxidoreductase enzyme: The enzyme that […]
Enzyme inhibition and types of enzyme inhibitors
What is enzyme inhibition? Enzyme inhibitors are the substance which when binds to the enzyme reversibly or irreversibly, decreases the activity of enzyme and the […]
Protein purification and characterization
Protein purification Purity is defined by the general level of protein contaminants and also by the absence of contaminants of special interests such as microbes, […]
Mass spectrometry Principle, instrumentation and applications
Introduction of mass spectrometer: In the technique of mass spectrometry, the compound under investigation is bombarded with a beam of electron which produces an ionic […]
Electrophoresis Principle, affecting factors and types
What is electrophoresis? The term electrophoresis describes the migration of a charged particles under the influence of an electric field. Various essential biological molecules, such […]
Concept of Disease causation in epidemiology and management of disease
Disease causation: Any event or condition, characteristics or combination of these factor which plays an important role in producing the disease cause may not be […]
Prion disease (an infectious protein)
What are prions? Prions are unusual proteinaceous infectious particles that cause a group of universally fatal neurodegenerative disease called the spongiform encephalopathies by an entirely […]
Fluorimetry-Principle and Applications
Introduction: A large number of substances are unknown which can absorb ultraviolet or visible light energy. But these substances lose excess energy through heat through […]