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Month: August 2020

Paper chromatography - Principle, Procedure, types and applications

Paper chromatography – Principle, Procedure, types and applications

August 13, 2020 Gaurab Karki 0

Principle of paper chromatography: This technique is a type of partition chromatography in which the substances are distributed between two liquids, i.e., one is the […]

Microbial Pectinase: industrial production and application

Microbial Pectinase: industrial production and application

August 13, 2020 Gaurab Karki 0

Pectinase enzyme: Pectinase is the collective term for row of enzyme that are able to breakdown or transform pectins. Pectinase is a general form for […]

Microbial Chitinase: production process and applications

Microbial Chitinase: production process and applications

August 12, 2020 Gaurab Karki 0

Chitinase: Chitinases are hydrolytic enzymes with the size ranging from 20kDa to about 90kDa. Chitinase present in wide range of organisms such as bacteria, fungi […]

Microbial Protease enzyme: industrial application and production process

Microbial Proteases: industrial application and production process

August 12, 2020 Gaurab Karki 0

Enzymes- Introduction Enzymes are proteins which catalyzes specific biochemical reactions is a very efficient manner. Enzymes can be produced by different types of micro-organisms, plant […]

Shigella: antigenic structure, cultural characteristics and biochemical tests

Shigella: antigenic structure, cultural characteristics and biochemical tests

August 12, 2020 Gaurab Karki 0

Genus: Shigella Dysentery is a clinical condition of multiple etiology characterized by frequent passage of blood-stained mucopurulent stool. The causative agent of bacillary dysentery (Disease […]

Microbial α and β amylase- Production process and industrial applications

Microbial α and β amylase- Production process and industrial applications

August 11, 2020 Gaurab Karki 0

Amylase enzyme: Starch, a glucose polymer is one of the most widely available plant polysaccharides which is hydrolysed by an enzyme called amylase. One of […]

Salmonella: morphology, antigenic structure, cultural and biochemical characteristics

Salmonella: morphology, antigenic structure, cultural and biochemical characteristics

August 11, 2020 Gaurab Karki 0

Genus Salmonella: Salmonella is an enterobacteria (fermentative, facultative anaerobes, oxidase -ve, gram -ve rods, catalase +ve). Motile (generally), aerogenic, non-lactose fermenting urease -ve, citrate +ve, […]

Single cell protein (SCP): Substrate and steps involved in production

Single cell protein (SCP): Substrate and steps involved in production

August 9, 2020 Gaurab Karki 0

Single cell protein: The dried cells of micro-organisms (Algae, Bacteria, Actinomycetes and fungi) used as food or feed are collectively called microbial protein. The term […]

Primary cell culture: Preparation of primary chick embryo fibroblast (CEF) culture

Primary cell culture-Preparation of primary chick embryo fibroblast (CEF) culture

August 9, 2020 Gaurab Karki 0

What is primary cell culture? The stage of the culture after isolation of the cells but prior to the first sub culture after which it […]

Oxidative phosphorylation: Electron transport chain and ATP synthesis

Oxidative phosphorylation: Electron transport chain and ATP synthesis

August 8, 2020 Gaurab Karki 0

Oxidative phosphorylation: Reducing equivalent NADH, FADH2 generated during glycolysis and the link between glycolysis and Kreb’s cycle are used to synthesize ATP by a process […]

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