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Month: August 2020

Microbiological assay and methods

Microbiological assay and methods

August 22, 2020 Gaurab Karki 0

Microbial assay Microorganisms have found widespread uses in the performance of bioassays for: Determining the concentration of certain compounds (e.g., amino-acids, vitamins and some antibiotics) […]

Rabies virus: Properties, life cycle, pathogenesis, diseases and diagnosis

Rabies virus: Properties, life cycle, pathogenesis, diseases and diagnosis

August 22, 2020 Gaurab Karki 0

What is Rabies? Rabies virus is also known as street virus. It causes rabies which is an acute infection of CNS and is always fatal […]

Beta Oxidation of fatty acid

Beta Oxidation of fatty acid: steps and examples

August 21, 2020 Gaurab Karki 0

Fatty acids Oxidation Oxidation of fatty acid (FA) releases large amount of energy. Oxidation of fatty acid occurs in mitochondria cell lacking mitochondria (e.g. RBC) […]

Methods of Cell disruption: Cell lysis methods

Methods of Cell disruption: Cell lysis methods

August 21, 2020 Gaurab Karki 0

Cell disruption: The process of breaking apart of the cells in order to obtain the desired product which may be intracellular or periplasmic is called […]

Enzyme classification with examples

Enzyme classification with examples

August 20, 2020 Gaurab Karki 0

Classification of enzyme: on the basis of International Union of Biochemistry (IUB) system: Oxidoreductase Transferase Hydrolase Lyase Isomerase Ligase 1. Oxidoreductase enzyme: The enzyme that […]

Enzyme inhibition and types of enzyme inhibitors

Enzyme inhibition and types of enzyme inhibitors

August 20, 2020 Gaurab Karki 0

What is enzyme inhibition? Enzyme inhibitors are the substance which when binds to the enzyme reversibly or irreversibly, decreases the activity of enzyme and the […]

Protein purification and characterization

Protein purification and characterization

August 19, 2020 Gaurab Karki 0

Protein purification Purity is defined by the general level of protein contaminants and also by the absence of contaminants of special interests such as microbes, […]

Mass spectrometry-Principle, instrumentation and applications

Mass spectrometry Principle, instrumentation and applications

August 18, 2020 Gaurab Karki 0

Introduction of mass spectrometer: In the technique of mass spectrometry, the compound under investigation is bombarded with a beam of electron which produces an ionic […]

Electrophoresis Principle, affecting factors and types

Electrophoresis Principle, affecting factors and types

August 17, 2020 Gaurab Karki 0

What is electrophoresis? The term electrophoresis describes the migration of a charged particles under the influence of an electric field. Various essential biological molecules, such […]

Concept of Disease causation in epidemiology and management of disease

Concept of Disease causation in epidemiology and management of disease

August 17, 2020 Gaurab Karki 0

Disease causation: Any event or condition, characteristics or combination of these factor which plays an important role in producing the disease cause may not be […]

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