Composition of Butzler campylobacter medium:

To make about 38 plates:

• Brucella agar base (oxoid)…………500 ml (thioglycollate medium with 20 g agar added, Columbia agar or oxoid blood agar base can be used)
• Sterile sheep blood……………………..75 ml
• Rifampicin……………………………………5 mg
• Colistin…………………………………………5000 IU
• Cafoperazone……………………………….7.5 mg
• Amphotericin B……………………………..1 mg

Preparation procedure of Butzler campylobacter medium

• Prepare and sterilize the Brucella agar or other base medium as instructed by the manufacturer. Transfer to a 50°C water bath.
• When the medium has cooled to 50°C add aseptically the sterile sheep blood (prewarmed to room temperature), and mix gently.
• Add the antibiotics and mix gently but well. Avoid forming air bubbles
• Dispense aseptically in sterile petri dishes as described.
• Label the plates
• Date the medium and give it a batch number.

Quality control

• pH of medium: depending on the agar base used, the pH should be within the range pH 7.2-7.5 at room temperature.
• Performance: test the medium as described for the control of a selective medium.
• *Store the plates at 2-8°C in sealed plastic bags to prevent loss of moisture.

Uses of Butzler campylobacter medium:

• Used as selective medium for isolation of Campylobacter jejuni and C. coli

Butzler campylobacter medium: composition, preparation and uses

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